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The effect of metallic substitution around the catalytic constants for NagA is usually utilized to evaluate the contributions made by these divalent cations on enzymatic activity (22, 23). The relationship on the worth of k

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For the single histidine substitutions, the N61H mutant is comparable in catalytic activity on the double mutant Whilst the Q59H mutant is diminished by less than an get of magnitude relative to the wild variety enzyme. To the substitutions at Asn-61, the alternative by having an alanine is substantially fewer disruptive compared to improve into a histidine.

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Residues Q59 and N61 ended up mutated alongside one another to a pair of histidine residues to produce an HxH motif that is analogous to that located in NagA from B. subtilis